ˎƷQ
ͨQײw\ԇУø(lin)߷
ӢQDiagnostic Kit for Antibody to Hepatitis C Virus (ELISA)
hZ(y)ƴBingxing Ganyan Bingdu Kangti Zhenduan Shijihe (Meilianmianyifa)
bҎ(gu)
48˷/С96˷/576˷/
A(y);
ԇвELISAzy(c)ѪѪ{(bio)wmѪҺĺY鼰RײȾo\
z(yn)ԭ
ELISAgӜy(c)A(y)бοԭķ(yng)׃(ni)y(c)(bio)(bio)HCVtװγɿԭ-w(f)wcø(bio)IgGY(ji)γɿԭ-w-ø(bio)w(f)(jng)@ɫϵy(tng)@ɫ(j)OD ֵжПo(w)HCVwĴڡ
ҪMɳ
ԇнMɣ |
48˷/ |
96˷/ |
576˷/ |
|||
1.A(y)(yng) |
48/K |
1K |
96/K |
1K |
96/K |
6K |
2.øY(ji) |
5ml |
1ƿ |
10ml |
1ƿ |
70ml |
1ƿ |
3.(yng)Ԍ(du) |
0.5ml |
1ƿ |
0.5ml |
1ƿ |
4ml |
1ƿ |
4.Ԍ(du) |
0.5ml |
1ƿ |
0.5ml |
1ƿ |
4ml |
1ƿ |
5.sϴҺ |
30ml |
1ƿ |
50ml |
1ƿ |
250ml |
1ƿ |
6.@ɫA |
3ml |
1ƿ |
6ml |
1ƿ |
40ml |
1ƿ |
7.@ɫB |
3ml |
1ƿ |
6ml |
1ƿ |
40ml |
1ƿ |
8.KֹҺ |
3ml |
1ƿ |
6ml |
1ƿ |
40ml |
1ƿ |
9.ƷϡҺ |
5ml |
1ƿ |
10ml |
1ƿ |
70ml |
1ƿ |
10. z |
2Ƭ |
2Ƭ |
12Ƭ |
|||
11. f(shu) |
1 |
1 |
1 |
|||
12.Է |
1ֻ |
1ֻ |
6ֻ |
ע⣺̖ͬ(ho)ԇԇнM12 ɻá
(ch)lЧڡ
1(ch)l28
2Чڣ12(g)
mÃx
1 Һ͝^
2 37ˮԡ
3 Ӌ(j)r(sh)
4 ϴC(j)
5 ʎ
6 ø(bio)x z(yn)L(zhng)450nmL(zhng)630nm
ӱҪ
1ӱɼҪ(zhn)䣬IJѪg(sh)ռѪҺȫѪƷȷ37̎2Сr(sh)ٌѪƷx3000D(zhun)/x10ϣʹѪѪ{OٺѪ(x)鿹ѪƷ(yng)ѪƷxĺٷҜ2Сr(sh)
2ӱʹѪѪ{ػEDTAȿ(hu)Ӱ푌(sh)(yn)Y(ji)
3ӱкïBc(hu)Ӱ푌(sh)(yn)Y(ji)߶ѪĘӱ](mi)ȫտsѪӱȾĘӱܕ(hu)e(cu)`ĽY(ji)
4rĘӱ28o(w)l±һrӱL(zhng)ڃ(ch)-20ⷴ(f)
z(yn)
1ÿԇ(yn)O(sh)(yng)Ԍ(du)ոɿքe(yng)Ԍ(du)100lO(sh)һհ(du)գӘƷϡҺ100l100lƷϡҺټӴy(c)Ѫ10lֻ3760ȥ׃(ni)Ʒĸ
2ϴ壺1ֹϴ壺ȥ(yng)l׃(ni)ҺwˮĸϴҺעMÿo5-10룬ȥ׃(ni)ϴҺĸ˷(f)5ĸɡ
2ϴC(j)ϴ壺xϴ5εijϴϴҺ(yng)עMÿף_ÿo(w)ˮĸɡ
3ÿøY(ji)100lհ(du)տ׳⣩3730ͬϷϴ(yng)5Ρ
4ÿ@ɫAҺBҺ50lppʎ37ܹo30
5ÿKֹҺ50lKֹ(yng)Կհ{(dio)ø(bio)xxȡOD450
ֵ
1Ԍ(du)ƽֵС0.1(yng)Ԍ(du)ƽֵ1.2(sh)(yn)Y(ji)Ч
2(sh)(yn)O(sh)Ӌ(j)Ҫ(yng)ԡԌ(du)ODֵ֮(yng)1.2tΌ(sh)(yn)o(w)Ч
3Ԍ(du)x(sh)С0.050r(sh)0.050Ӌ(j)㡣
4RֵCut off value=Ԍ(du)ƽֵ+0.1
z(yn)Y(ji)ጡ
1y(c)ԇ(bio)ODֵСCut off valuetHCVwy(c)ԇ(bio)ODֵڻCut off valuetHCVw(yng)
2ԽY(ji)ӱв-HCVӱе-HCVԇеęzy(c)
3(yng)ԽY(ji)ӱк-HCVخ(yng)
4(yng)ԽY(ji)ĘӱҪM(jn)؏(f)(sh)(yn)؏(f)Y(ji)(yng)ԵĘӱ(yng)(jng)^(gu)m(dng)?sh)ֶM(jn)д_J(rn)
1 ڃxӘɵĽȾ
2 @ɫxȾ
3 ԇ©ɵĽȾ
4 ֵϴȥص
ԇ(yn)ľԡ
ƷHڙzy(c)ѪѪ{-HCVwzy(c)Ro\
a(chn)Ʒָ(bio)
1zy(c)30HCVw(gu)ԅƷ(yng)Է(yng)ö1ݣR29/30
2zy(c)30HCVw(gu)(yng)ԅƷԷ(yng)ö1ݣR29/30
34HCVw(gu)͙zޅƷ(yng)Է(yng)2һ|(zh)Ѫ
41HCVw(gu)ҾԅƷM(jn)ЙzCV(%)(yng)15%(n=10)
5(wn)Ԍ(sh)(yn)ԇM376zY(ji)(yng)_(d)(bio)(zhn)
ע(xing)
1 ƷHw\࣬Hڙzy(c)wѪѪ{zy(c)Y(ji)\ָʾ֮һ
2 28lȡĘӱ(yng)Ҝƽ30M(jn)Йzy(c)
3 횴_ƷӘĜ(zhn)_ӘӲ(zhn)_ܕ(hu)(do)e(cu)`Č(sh)(yn)Y(ji)
4 ʹҺֹӘӕr(sh)ÿΑ(yng)ԓQ^ȡӱ
5 ڲ^(gu)Б(yng)Mⷴ(yng)Юa(chn)
6 sϴҺϵߝ}ܕ(hu)γɽY(ji)δȫܽ⣬(hu)Ӱ푌(sh)(yn)Y(ji)ͬr(sh)ܕ(hu)סԄ(dng)ӘϴC(j)ܵF(xin)Y(ji)Ո(qng)37ܽȻϡጻʹá
7 z؏(f)ʹ
8 ˮԡ偷(yng)r(sh)Ո(qng)(yng)ˮ1/3ײԾW(wng)֧֧ˮؿ37
9ϴ壺
1ϴC(j)ϴr(sh)ϴC(j)ļҺP(gun)ҪȱϴҺ^(gu)ܳM(yng)ϴΔ(sh)(yng)ԓ5Σ(jng)עzҺ^Ƿ
2ֹϴr(sh)Ո(qng)ʹÎмˮİ壬ԷԴ^(gu)ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zy(c)Y(ji)Ĝ(zhn)_
3ϴr(sh)õˮՈ(qng)(f)ʹá
4ϴC(j)ÿʹǰsˮȥxˮϴɃԷֹܵg
10ʹȫԄ(dng)ø(bio)ϵy(tng)ܕ(hu)ϵy(tng)`(yng)(du)ϵy(tng)(sh)O(sh)m(dng){(dio)ԝM(gu)P(gun)|(zh)ƷҪ
11κԇ|Ƥw۾ôˮ(du)ԓλM(jn)ДU(ku)ϴKֹҺиgԡ
12ԇЃ(ni)P(gun)MݼRӱҕНڂȾՈ(qng)P(gun)Č(sh)(yn)ҹҎ(gu)(li)(zh)к̎