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ͬͰװyԇ_ϺsʢˎI(y)ɷ޹˾
ԇ
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ͬͰװyԇ

ͬͰװyԇ

a(chn)ƷQ

ͨQͬͰװyԇУø

bҎ(gu)

ԇ1R1132mlԇ2R2110.5ml

ԇ1R1220mlԇ2R2113ml

ԇ1R1232mlԇ2R2210.5ml

4x60TR1163.8ml R282.2ml

8x60TR1323.8ml R2162.2ml

A(y);

t(y)C(j)(gu)ѪͬͰװȵwzyo\

z(yn)ԭ

СӲ@g(sh)SMTS-HcySAHˮøķ--һ좣TCEPHcyD(zhun)xHcyͨ^cr(ji)S-ռᷴ(yng)ɵSAHSAHSAHˮøˮպHcyHcy(jng)ѭh(hun)(yng)Ŵ̖ͬr(sh)a(chn)ˮɰʹSڹȰÓø£ʹNADHD(zhun)NAD+ӱеHcycNADHD(zhun)ʳ

ҪMɳɷ֡

ԇ1

S-ռᣨSAM 0.1mM

NADH > 0.2 mM

TCEP > 0.5 mM

2-ͪ 5.0 mM

ԇ2

ȰÓø 10KU/L

SAHˮø 3.0 KU/L

Óø 5.0 KU/L

Hcy ׻D(zhun)ø 5.0 KU/L

ͬԇûá

lЧڡ

ԇa(chn)ܹ֮]2-8oCЧڞ12xϵ_ƿԇԷ(wn)30

a(chn)ڼЧҊ(bio)

mÃx

a(chn)ƷҪm_ʽȫԄxhÑЃxʹña(chn)Ʒǰ(j)(sh)(yn)rM(jn)(yn)C

1HITACHI 7060708071807600̖_ʽȫԄx

2OLYMPUS AU 400AU640AU2700AU5400̖_ʽȫԄx

3Abbott C8000̖_ʽȫԄx

4Roche Modular P800̖_ʽȫԄx

5BECKMAN DXC800̖_ʽȫԄx

6~400800̖_ʽȫԄx

7TRXL-MAX̖ȫԄx

8|֥120̖_ʽȫԄx

9|ܛ400̖_ʽȫԄx

10Bayer ADVIA 1650/2400̖_ʽȫԄx

ӱҪ

rѪ壬ӱռ֮ҪxķxѪ粻xռõĘӱ(yng)ԓڱб棬һСr(sh)(ni)xѪ֬ѪĘӱhʹ(jng)^xѪӱҜؿԱ408ܱڣ20ׂܱ

z(yn)

1.ԇ(yn)lL340nmɫ⏽1.0cmض37

2.ԇ(yn)

  հ У(zhn)Ʒ ӱ
 ԇ1 240 240 240
 հףl 13    
 У(zhn)Ʒl      
 ӱl      
37ԡ5min
 R2l 65 65 65
37ԡ2.5 minxȡA1
37ԡ2.5 minxȡA2

A=[(A2-A1)У(zhn)ƷܻƷ]-[(A2-A1)հ׹]

7060ȫԄx(sh)O(sh)£


ض 37 (yng) c(din)ʷ
L/L 700nm/340nm
R1/s/R2
240l/13l /65l
MϷʽ Spline (yng) ½
A1yc(din)
24 A2yc(din) 31

 

  3. У(zhn)|(zh)Ƴ

ڌӱM(jn)Йzyr(sh)ʹƷԴԵУ(zhn)ƷM(jn)ж(bio)ٌƷԴԵ|(zh)ƷM(jn)ЙzyzyY(ji)S(ni)܌ӱM(jn)Йzy

4. ԇ(yn)Y(ji)Ӌ(j)㷽

ͬͰװУ(zhn)ƷĘ(bio)ʾȞM(bio)XͬͰװУ(zhn)Ʒ(sh)HyֵAv(bio)yM(jn)зǾԔMsplinelogitȣLƘ(bio)(zhn)yӱĜyֵA(bio)(zhn)ɵó(yng)yӱͬͰװᣨHcyĝȣmol/L

^(q)g

15mol/Lh(sh)(yn)Ҵ_(w)Ⱥą

z(yn)Y(ji)Ľጡ

ӱѪt1200mg/dLđtء20mg/dL֬2500mg/dLSC10mmol/Lѡ20mol/Lr(sh)yoɔ_

z(yn)ľԡ

1. ԇľԷ050mol/L(dng)ӱͬͰװȸ50mol/Lr(sh)(yng)ԓˮϡጺٜyY(ji)(yng)ϡጱ(sh)

2. ԇ2~8oCɃ

3. ԇ(yng)ԓ峺׃ʼС0.800A340nm⏽1.0cm(yng)ԓG

 4. S-ͬͰװ@ɔ_wѪ{кSAHOyԇY(ji)ɸɔ_

5. ˿?yn)zijЩˎͬͰװߣװRƽӢ@ʄ

6. ܙzy3-żյĘӱ

a(chn)Ʒָ(bio)

1^ȻlM(jn)Ŀҕzya(chn)ƷҪԭϵ^Ҫ£

ԇ1oɫҺw

ԇ2oɫSɫҺw

2 ݘ(bio)ʾ

3ԇհȣ340nm37&1.0cm⏽0.800A

4`ȣԇyԇ10mol/LͬͰװӱr(sh)ȲֵA0.01A

5ԣ050mol/L(ni)

a)P(gun)ϵ(sh)r0.995

b) ƫ^10%^ƫ(yng)^1.0mol/Lc(din)Ӌ(j)ã

6ܶȣ

6.1؏(f)CV5.0%ӱȞ10.02.0mol/LCV3.0%ӱȞ20.04.0mol/L

6.2gO10%ӱȞ10.02.0mol/L

7(zhn)_ȣyԇur(ji)Ҏ(gu)ą|(zh)Y(ji)c(bio)ʾֵƫ(yng)^15%

8(wn)ԣԇa(chn)ܹ֮⣬]2~8ɷ(wn)12¡

ע(xing)

1. ԇЃHw\ʹ

2. ̖ͬԇûá

3. ԇкзBcՈֱӽ|Ƥw۾һ|ôˮ_ϴՈ̷

4. ԇеĜyY(ji)HNRo\(j)

5. ԇ͘ӱɸ(j)xIJͬ׃

6. У(zhn)ƷmȻ䝓ڵΣU(xin)ѽ(jng)̎^谴Դӱ

7. У(zhn)ƷкиͣȫԄӷxҪճϴM(jn)ϴԇwԇϡ

īI(xin)

1w\ԇRоg(sh)ָ(do)ԭtԇУ

2R(sh)(yn)҅Ķxʹ_

3 2006|ϴW(xu)ġȫRz(yn)Ҏ(gu)̡

4RW(xu)w\ԇУͨüg(sh)Ҫ