a(chn)ƷQ
ͨQTᰱD(zhun)øy(c)ԇУBm(x)O(jin)y(c)
bҎ(gu)
|
Ҏ(gu)1 |
Ҏ(gu)2 |
Ҏ(gu)3 |
Ҏ(gu)4 |
Ҏ(gu)5 |
Ҏ(gu)6 |
ԇ 1 |
240ml | 460ml | 280ml | 450ml | 440ml | 460ml |
ԇ 2 |
210ml | 230ml | 220ml | 225ml | 220ml |
160ml |
A(y);
t(y)C(j)(gu)wy(c)Ѫ壨Ѫ{ӱTᰱD(zhun)øĻo\á
ѪеTD(zhun)øVڸKļǸ켡IX(x)ͼt(x)@ЩMpr(sh)TD(zhun)ø@ЩMጳʹѪTD(zhun)ø
z(yn)ԭ
ӱеASTL-Tͦ-ͪᰱD(zhun)QɲL-ȰNADHOÓøMDH£ᱻ߀ԭL-ONADHNAD+Ķʹ340nm̎Ĺֵ½ͨ^O(jin)y(c)340nmֵ̎½ʣԜy(c)AST
ҪMɳɷ֡
R1TrisTrisHClNADH0.3g/LLDH MDH1.2KU/L
R2TrisL-Tᣨ200g/L-ͪ
̖ͬ(ho)ԇиMݲԻQ
(ch)lЧڡ
ԇa(chn)⃦ܹ(ch)28ɷ(wn)12(g)
a(chn)ڼʧЧҊ(bio)
mÃx
a(chn)ƷҪm_ʽȫԄ(dng)xhÑЃxʹña(chn)Ʒǰ(j)(sh)(yn)rM(jn)(yn)C
Abbott Aeroset/Architect c8000
BeckmanCoulterSynchronCX3/CX4/CX5/CX7/LX20
Bayer ADVIA 1200/1650/2400
HITACHI7020/7060/7060C/7070/7080/7150/7170/7170S/7170A/7180/7600
Konelab 30/60i
OLYMPUS AU 400/600/640/2700/5400
Roche Cobas Mira Plus Roche MODULAR P800/D2400
SHIMADZU CL7200/7300/8000
VITALAB Selectra-XL/E
TOSHIBA 30/30FR/40FR/120FR
~ OmilabTM BS-300/400
Sysmex CHEMIX-180
2000
ӱҪ
ѪѪASTҜأ20棩Ա48Сr(sh)4¿Ա1-25¿Ա1(g)¡(yn)֬ѪSѪѪܕ(hu)y(c)
z(yn)
1.ԇƣԇֱʹ
2. ԇ(yn)l
|
37 |
|
10l |
L |
340nm |
ԇR1/R2 |
200/50l |
tr(sh)g |
60 |
y(c)ģʽ |
ʷ |
O(jin)y(c)r(sh)g |
120 |
(yng) |
½ |
3У(zhn)|(zh)Ƴ
@(g)(sh)(yn)ҪУ(zhn)Ʒڌ(du)ӱM(jn)Йzy(c)r(sh)ʹƷԴԵ|(zh)ƷM(jn)Йzy(c)zy(c)Y(ji)S(ni)܌(du)ӱM(jn)Йzy(c)
4ԇ(yn)Y(ji)Ӌ(j)㷽
Kӣ |
TV1000 |
6.22SVP |
ASTU/LA/minK
ASTU/LA/min4180
ʽ TV(yng)weml SVӱweml
6.22=NADH340 nm̎ĺĦϵ(sh) Pɫ⏽cm
KڲͬxϿвͬh(sh)(yn)ҽԼKӡ
^(q)g
<42U/L (37) õąֵֵHh(sh)(yn)(yn)C@һԼąֵ
z(yn)Y(ji)Ľጡ
Ѫtס4g/LSC0.4g/Lɔ_Y(ji)
z(yn)ľԡ
(dng)z(yn)Y(ji)800U/Lr(sh)ҪѪӱϡጺ(f)
a(chn)Ʒָ(bio)
1. ԇ^R1: oɫSɫҺwR2: oɫSɫҺw
2. ݘ(bio)ʾ
3. ԇհȣ1.0 AL340nm⏽1.0cmԇհ׃ʣ0.004A/min
4. `ȣԇy(c)ԇ100U/LASTӱr(sh)׃ֵA0.012A/min
5. Է0800U/L(ni)Ӌ(j)-(yng)P(gun)ϵ(sh)r0.990ƫܡ15%^(du)ƫ8U/Lc(din)Ӌ(j)ã
6. y(c)ܶȣ؏(f)ԑ(yng)5%g(yng)5%
7. (zhn)_ȣy(c)ԇu(png)r(ji)Ҏ(gu)C|(zh)Y(ji)c(bio)ʾֵ(du)ƫ(yng)ܡ10%
8. ԇķ(wn)ԣԇa(chn)⃦ܹ(ch)2~8ɷ(wn)ʮ(g)£(xing)g(sh)ָ(bio)g⣩ڷ֮(ni)
ע(xing)
1. ֬S(bio)340nm̎^(qing)շ壬@Щ(bio)иˮƽAST(hu)(do)µıM340nmȻSָ߹ֵ˕r(sh)Ʒ(yng)ԓϡጺٜy(c)ԇ
2. ԇHw\࣬зNaN3ܿ
īI(xin)
1. IFCC methods for the measurement of catalytic enzymes. J Clin Chem Clin Biochem 1986; 24:497-510
2. Gella FJ, et al. Clin Chim Acta 1985; 241-257
3. Young, D.S.et al. Clin Chem 1975; 21:5