a(chn)ƷQ
ͨQײĿwzyԇУø(lin)߷
bҎ(gu)
48˷/96˷/576˷/
A(y);
a(chn)ƷwⶨԙzyѪѪ{ײĿw
ײHBVȾR\ָ(bio)֮һ
zԭ
ײĿwzyԇвøƷzyҸκĿw-HBcûؽMҸκĿԭHBcAglHRP(bio)ӛĿ-HBcø(bio)ӛļ(lin)TMB^y(bio)ͬr뿹-HBc-HRPcԭγɸY(ji)ϣ(dng)(bio)п-HBcߕrt-HBc-HRPcҸκĿԭHBcAgY(ji)TMBr@ɫ֮t@ɫڌY(ji)roɫɫ׃ʾҸκĿw-HBc@ɫģtʾoҸκĿw-HBc
ҪMɳɷ֡
ԇнM |
48˷/ |
96˷/ |
576˷/ |
1.A(y)(yng) |
48/K1K |
96/K1K |
96/K6K |
2.øY(ji) |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
3.Ԍ |
0.5mL1ƿ |
0.5mL1ƿ |
2mL1ƿ |
4.Ԍ |
0.5mL1ƿ |
0.5mL1ƿ |
2mL1ƿ |
5.sϴҺ |
30mL1ƿ |
30mL1ƿ |
100mL2ƿ |
6.@ɫA |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
7.@ɫB |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
8.KֹҺ2M H2SO4 |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
9.z |
1Ƭ |
1Ƭ |
6Ƭ |
10.Է |
1ֻ |
1ֻ |
6ֻ |
ע⣺̖ͬԇԇнM12 ɻ
MݽMɣ
øY(ji)Na2HPO412H2O 6.7g/LNaH2PO42H2O 0.16g/LKCl 0.2g/LNaCl 8g/LwAøo0.6g/LСţѪ 50mL/LҺwAҺw7mL/Lָʾ1 1.25mL/LHBcAb-HRPm
ԌգNa2HPO412H2O 2.9g/LNaCl 8g/LKCl 0.2g/LKH2PO4 0.2g/LNaN3 60g/LҶ 200mL/LСţѪ 200mL/Lָʾ1 1.25mL/LѪm
ԌգNa2HPO412H2O 6.7g/LNaH2PO42H2O 0.16g/L KCl 0.2g/L NaCl 8g/LҺwAҺw 5mL/Lָʾ2 0.55mL/LѪ 200mL/L
sϴҺNa2HPO412H2O 58g/L NaCl 160g/LKCl 4g/LKH2PO4 4g/LTween-20 10 mL/L
@ɫA 8g/LԇA 0.004g/LԇB 2.4mL/L^ 0.7g/L
@ɫB 2.1g/LҶc 0.38g/LTMB 0.4g/L
KֹҺ 2M H2SO4
ҪδṩƷ
1. rsˮȥxˮ
2. һ
3. _Һ͝^
4. Ƀˮ
5. 37ˮԡ
6. Ӌr
7. ϴCϴƿC
8. ʎ
lЧڡ
ԇБ(yng)28бܹⱣ棬Ч12
a(chn)ڼЧҊ(bio)
mÃx
ø(bio)x zyL450nmL630nm
ӱҪ
1. ӱɼҪ(zhn)䣬Ҫܙz߽ʳIJѪg(sh)ռѪҺȫѪƷȷ37̎2СrٌѪƷx3000D(zhun)/x6ʹѪ壨{OٺѪ鿹ѪƷ(yng)ѪƷxĺٷҜ2Сr
2. ӱʹѪѪ{ػEDTAȿӰ푌Y(ji)
3. ӱкïBcӰ푌ĽY(ji)߶ѪĘӱ]ȫտsѪӱȾĘӱܕe`ĽY(ji)
4. rĘӱ28ol¿ɱһڣrӱLA-20ⷴ(f)ڡ
z
1. (zhn)䣺حh(hun)ȡԇҜƽ30ͬrsϴҺ120ϡጡ
2. Ӵy(bio)y(bio)ÿ0.05yӱ1:30ϡጣO(sh)Ԍ2ףԌ20.05հ1
3. øY(ji)ÿ0.05հտײֻNz3730
4. ϴ壺1ֹϴ壺ȥ(yng)l׃(ni)ҺwˮĸɣϴҺעMÿo510ȥ׃(ni)ϴҺĸ˷(f)5ĸ
2ϴCϴ壺xϴ5εijϴϴҺ(yng)עMÿ_ÿoˮĸɡ
5. @ɫ ȼ@ɫAÿ0.05 ټ@ɫBÿ0.05ֻ37ܹ15
6. Kֹ(yng) ÿKֹҺ0.05
7.yø(bio)xx(sh)xβL450 nmԿհУ㣩pL450/630 nmxȡODֵx(sh)ڽKֹ(yng)10犃(ni)ɡ
Дֵ
Cutoff ֵӋ㣺 ԭѪCOV = ԌƽODֵ0.3
1:30ϡѪCOV = ԌƽODֵ0.5
(bio)ODֵCOV(bio)ODֵCOV
zY(ji)Ľጡ
1. ԽY(ji)ӱвҸκĿw-HBcӱеҸκĿw-HBcԇеęzy
2. ԽY(ji)ӱкҸκĿw-HBcخ(yng)ء
3. ԽY(ji)ĘӱҪM؏(f)ֻн(jng)^؏(f)؏(f)ԞԽY(ji)ĘӱJҸκĿw-HBc؏(f)Y(ji)ԵĘӱ(yng)(jng)^m(dng)?sh)ֶMд_J
ԽY(ji)Ęӱ(f)zԽY(ji)(yng)JԘӱ؏(f)ԽY(ji)܌һg(sh)}£
1 ڃxӘɵĽȾ
2 @ɫxȾ
3 ԇ©ɵĽȾ
4 ֵϴȥص
4. Ѫzy͵ζҸκĿw-HBcֻȾвW(xu)xֻЙzyߵζȿw1:30Bm(x)zywζuߕrҸκĿw-HBcſȾָ(bio)
zľԡ
иԵߌϵy(tng)ڝڵķخԷ(yng)؏(f)ԽY(ji)(yng)һm(dng)?sh)ķMд_J
a(chn)Ʒָ(bio)
1 ԇ^Һwo
2 ԅƷʣ15ԅƷzyY(ji)óF(xin)
3 ԅƷʣ15ԅƷSF(xin)1ݼ
4 ͙z͙zƷy(yng)1IU/mL
5 ԣþԅƷyCV(yng)20n=10
6 (wn)ԇԇM376zY(ji)(yng)_(bio)(zhn)15
7 bݘ(bio)ʾb
ע헡
1. ƷHw\࣬HޙzywѪѪ{zyY(ji)\ָ(bio)֮һ
2. 28lȡĘӱ(yng)Ҝƽ30MЙzy
3. 횴_ƷӘĜ(zhn)_ӘӲ(zhn)_ܕ(do)e`ČY(ji)
4. ʹҺֹӘӕrÿΑ(yng)ԓQ^ȡӱ
5. õƿμӕrՈJҺwuȥ12κֱٵصμӡҪ⌢MеҺw|R߅ϣeøY(ji)
6. ڲ^Б(yng)Mⷴ(yng)Юa(chn)ݡ
7. sϴҺϵߝ}ܕγɽY(ji)δȫܽ⣬Ӱ푌Y(ji)ͬrܕסԄӼӘϴCܵF(xin)Y(ji)Ո37ܽȻϡጻʹ
8. z؏(f)ʹá
9. ˮԡ偷(yng)rՈ(yng)ˮ1/3ײԾW(wng)֧֧Σˮؿ37
10. ϴ壺1ϴCϴrϴCļҺP(gun)ҪȱϴҺ^ܳM(yng)УϴΔ(sh)(yng)5(jng)עzҺ^Ƿ
2ֹϴrՈʹÎмˮİ壬ԷԴ^ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zyY(ji)Ĝ(zhn)_
3ϴrõˮՈ(f)ʹá
4ϴCÿʹǰsˮȥxˮϴɃԷֹ·g
11. κԇ|Ƥw۾ôˮԓλMДUϴKֹҺиgԡ
12. ԇЃ(ni)P(gun)MݼRӱ(yng)ҕНڂȾՈP(gun)ČҹҎ(gu)(zh)к̎