a(chn)ƷQ
ͨQײeԭzyԇУø(lin)߷
bҎ(gu)
48˷/96˷/С576˷/
A(y);
ԇmwⶨԙzyѪѪ{ײeԭ
ײHBVȾR\ָ(bio)֮һ
z(yn)ԭ
ײeԭzyԇвpwAķzyҸeԭHBeAgÆοҸeԭ-HBelHRP(bio)ӛĿ-HBeø(bio)ӛļ(lin)TMB^(dng)(bio)дҸeԭHBeAgrԓҸeԭHBeAgc-HBeY(ji)ϲc-HBe-HRPY(ji)γɿ-HBe- HBeAg--HBe-HRP(f)TMBa(chn)@ɫ(yng)֮to@ɫ(yng)ڌ(sh)(yn)Y(ji)rɫ׃ʾҸeԭHBeAgڣoɫɫ׃tʾҸeԭHBeAg
ҪMɳɷ֡
ԇнM |
48˷/ |
96˷/ |
576˷/ |
1.A(y)(yng) |
48/K1K |
96/K1K |
96/K6K |
2.øY(ji) |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
3.Ԍ |
0.5mL1ƿ |
0.5mL1ƿ |
2mL1ƿ |
4.Ԍ |
0.5mL1ƿ |
0.5mL1ƿ |
2mL1ƿ |
5.sϴҺ |
30mL1ƿ |
30mL1ƿ |
100mL2ƿ |
6.@ɫA |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
7.@ɫB |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
8.KֹҺ2M H2SO4 |
3mL1ƿ |
6mL1ƿ |
40mL1ƿ |
9.z |
1Ƭ |
1Ƭ |
6Ƭ |
10.Է |
1ֻ |
1ֻ |
6ֻ |
ע⣺̖ͬԇԇнM12 ɻ
MݽMɣ
øY(ji)Na2HPO412H2O 2.9g/LKH2PO4 0.2g/LKCl 0.2g/LNaCl 8g/Lҵ 1g/LСţѪ 400mL/LҺwAҺw5mL/L 50mL/L ָʾ1 1.25mL/LTween-20 0.5mL/L HBeAb-HRP m
ԌգNa2HPO412H2O 2.9g/LNaCl 8g/LKCl 0.2g/LKH2PO4 0.2g/L 2g/LҶ 200mL/LСţѪ 200mL/Lָʾ1 1.25mL/L ؽMҸeԭHBeAgm
Ԍգ Na2HPO412H2O 2.9g/L NaCl 8g/LKCl 0.2g/LKH2PO4 0.2g/LNaN3 60g/Lָʾ2 0.555mL/LѪ 300mL/L
sϴҺ Na2HPO412H2O 58g/L NaCl 160g/LKCl 4g/LKH2PO4 4g/LTween-20 10mL/L
@ɫA 8g/LԇA 0.004g/LԇB 2.4mL/L^ 0.7g/L
@ɫB 2.1g/LҶc 0.38g/LTMB 0.4g/L
KֹҺ 2M H2SO4
ҪδṩƷ
1. rsˮȥxˮ
2. һ
3. _Һ͝^
4. Ƀˮ
5. 37ˮԡ
6. Ӌ(j)r
7. ϴC(j)ϴƿC(j)
8. ʎ
lЧڡ
ԇБ(yng)28бܹⱣЧ12
a(chn)ڼЧҊ(bio)
mÃx
ø(bio)x zyL450nmL630nm
ӱҪ
1. ӱɼҪ(zhn)䣬Ҫܙz߽ʳIJѪg(sh)ռѪҺȫѪƷȷ37̎2СrٌѪƷxģ3000D(zhun)/x6ʹѪ壨{OٺѪ(x)鿹ѪƷ(yng)ѪƷxĺٷҜ2Сr
2. ӱʹѪѪ{ᡢػEDTAȿӰ푌(sh)(yn)Y(ji)
3. ӱкïBcӰ푌(sh)(yn)ĽY(ji)߶ѪĘӱ]ȫտsѪӱȾĘӱܕe`ĽY(ji)
4. rĘӱ28ol¿ɱһrӱLA-20ⷴ(f)
z(yn)
1. (sh)(yn)(zhn)䣺حh(hun)ȡԇҜƽ30ͬrsϴҺ120ϡጡ
2. Ӵy(bio)y(bio)ÿ0.05O(sh)Ԍ2ףԌ20.05հ1
3. øY(ji)ÿ0.05հտײӣֻNz3730犡
4. ϴ壺1ֹϴ壺ȥ(yng)l׃(ni)ҺwˮĸϴҺעMÿo510ȥ׃(ni)ϴҺĸɣ˷(f)5ĸ
2ϴC(j)ϴ壺xϴ5εijϴϴҺ(yng)עMÿ_ÿoˮĸ
5. @ɫ ȼ@ɫAÿ0.05 ټ@ɫBÿ0.05ֻ37ܹ15犡
6. Kֹ(yng) ÿKֹҺ0.05
7. yø(bio)xx(sh)xβL450 nmԿհУ㣩pL450/630 nmxȡODֵx(sh)ڽKֹ(yng)10犃(ni)
Дֵ
Cutoff ֵӋ(j)㣺 COV = ԌƽODֵ2.1
(bio)ODֵCOV(bio)ODֵCOV
עԌODֵ0.050.05Ӌ(j)0.05(sh)HODֵӋ(j)
z(yn)Y(ji)Ľጡ
1. ԽY(ji)ӱвҸeԭHBeAgӱеҸeԭHBeAgԇеęzy
2. ԽY(ji)ӱкҸeԭHBeAgخ(yng)ء
3. ԽY(ji)ĘӱҪM(jn)؏(f)(sh)(yn)ֻн(jng)^؏(f)؏(f)(sh)(yn)ԞԽY(ji)ĘӱJ(rn)ҸeԭHBeAgԡ؏(f)Y(ji)ԵĘӱ(yng)(jng)^m(dng)?sh)ֶM(jn)д_J(rn)
ԽY(ji)Ęӱ(f)zԽY(ji)(yng)J(rn)Ԙӱ؏(f)ԽY(ji)܌һg(sh)}£
1 ڃxӘɵĽȾ
2 @ɫxȾ
3 ԇ©ɵĽȾ
4 ֵϴȥص
z(yn)ľԡ
иԵߌ(sh)(yn)ϵy(tng)ڝڵķخԷ(yng)؏(f)ԽY(ji)(yng)һm(dng)?sh)ķM(jn)д_J(rn)
a(chn)Ʒָ(bio)
1 ԇ^Һwo
2 ԅƷʣ15ԅƷzyY(ji)óF(xin)
3 ԅƷʣ10ԅƷzyԲö1
4͙z͙zƷy(yng)1IU/mL
5 ԣþԅƷyCV(yng)15n=10
6 (wn)ԇ(yn)ԇM376zY(ji)(yng)_(d)15Ҫ
7 bݘ(bio)ʾb
ע(xing)
1. ƷHw\HޙzywѪѪ{zyY(ji)\ָ(bio)֮һ
2. 28lȡĘӱ(yng)Ҝƽ30M(jn)Йzy
3. 횴_ƷӘĜ(zhn)_ӘӲ(zhn)_ܕ(do)e`Č(sh)(yn)Y(ji)
4. ʹҺֹӘӕrÿΑ(yng)ԓQ^ȡӱ
5. õƿμӕrՈJҺwuȥ12κֱٵصμҪ⌢MеҺw|?yn)R߅eøY(ji)
6. ڲ^Б(yng)Mⷴ(yng)Юa(chn)
7. sϴҺϵߝ}ܕγɽY(ji)δȫܽ⣬Ӱ푌(sh)(yn)Y(ji)ͬrܕסԄӼӘϴC(j)ܵF(xin)Y(ji)Ո37ܽȻϡጻʹ
8. z؏(f)ʹ
9. ˮԡ偷(yng)rՈ(yng)ˮ1/3ײԾW(wng)֧֧ˮؿ37
10. ϴ壺1ϴC(j)ϴrϴC(j)ļҺP(gun)ҪȱϴҺ^ܳM(yng)ϴΔ(sh)(yng)5Σ(jng)עzҺ^Ƿ
2ֹϴrՈʹÎмˮİԷԴ^ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zyY(ji)Ĝ(zhn)_
3ϴrõˮՈ(f)ʹ
4ϴC(j)ÿʹǰsˮȥxˮϴɃԷֹ·g
11. κԇ|Ƥw۾ôˮԓλM(jn)ДU(ku)ϴKֹҺᣬиg
12. ԇЃ(ni)P(gun)MݼRӱ(yng)ҕНڂȾԣՈP(gun)Č(sh)(yn)ҹҎ(gu)(zh)к̎