ˎƷQ
ͨQײ濹ԭ\ԇУø(lin)߷
ӢQDiagnostic Kit for Hepatitis B Virus Surface Antigen (ELISA)
hZƴYixing Ganyan Bingdu Biaomian Kangyuan Zhenduan Shijihe (Meilianmianyifa)
bҎ(gu)
48˷/96˷/576˷/
A(y);
ײ濹ԭ\ԇmڶԜyѪѪ{ײ濹ԭѪҺY鼰RײȾo\
z(yn)ԭ
ײ濹ԭ\ԇвpwAķzyHBsAgÿ-HBslHRP(bio)ӛĿ-HBsø(bio)ӛļ(lin)TMB^(dng)(bio)дHBsAgrԓHBsAgc-HBsY(ji)ϲc-HBs-HRPY(ji)γɿ-HBs-HBsAg--HBs-HRP(f)TMBa(chn)@ɫ(yng)֮to@ɫ(yng)ڌ(sh)(yn)Y(ji)rɫ׃ʾHBsAgoɫɫ׃tʾHBsAg
ҪMɳɷݡ
ԇнMɣ |
48˷/ |
96˷/ |
576˷/ |
|||
1.A(y)(yng) |
48/K |
1K |
96/K |
1K |
96/K |
6K |
2.øY(ji) |
3ml |
1ƿ |
6ml |
1ƿ |
40ml |
1ƿ |
3.Ԍ |
0.8ml |
1ƿ |
0.8ml |
1ƿ |
4ml |
1ƿ |
4.Ԍ |
0.8ml |
1ƿ |
0.8ml |
1ƿ |
4ml |
1ƿ |
5.sϴҺ |
30ml |
1ƿ |
30ml |
1ƿ |
100ml |
2ƿ |
6.@ɫA |
3ml |
1ƿ |
6ml |
1ƿ |
40ml |
1ƿ |
7.@ɫB |
3ml |
1ƿ |
6ml |
1ƿ |
40ml |
1ƿ |
8KֹҺ2MH2SO4 |
3ml |
1ƿ |
6ml |
1ƿ |
40ml |
1ƿ |
9.z |
2Ƭ |
2Ƭ |
12Ƭ |
|||
10.f |
1 |
1 |
1 |
|||
11.Է |
1ֻ |
1ֻ |
6ֻ |
ע⣺̖ͬԇԇнM12 ɻá
Ʒ̓x
1. rsˮȥxˮ
2. һ
3. _Һ͝^
4. Ƀˮ
5. 37ˮԡ
6. Ӌr
7. ϴC(j)ϴƿ
8. ʎ
9. ø(bio)x zyL450nmL630nm
lЧڡ
ԇБ(yng)28бܹⱣ棬Ч12
ӱҪ
1. ӱɼҪ(zhn)䣬Ҫܙz߽ʳIJѪg(sh)ռѪҺȫѪƷȷ37̎2СrٌѪƷx3000D(zhun)/x6ϣʹѪ壨{OٺѪ(x)鿹ѪƷ(yng)ѪƷxĺٷҜ2Сrϡ
2. ӱʹѪѪ{ᡢػEDTAȿӰ푌(sh)(yn)Y(ji)
3. ӱкïBcӰ푌(sh)(yn)ĽY(ji)߶ѪĘӱ]ȫտsѪӱȾĘӱܕe`ĽY(ji)
4. rĘӱ28olɱһrӱLA-20£ⷴ(f)
z(yn)
1. (sh)(yn)(zhn)䣺حh(hun)ȡԇҜƽ30ͬrsϴҺ120ϡ
2. Ӵy(bio)ÿԇ(yn)O(sh)ԡԌոɿքeԌ0.1հ1ףy(bio)0.1, 3760
3. øY(ji)ÿ0.05հտײֻ3730犡
4. ϴ壺1ֹϴ壺ȥ(yng)l׃(ni)ҺwˮĸϴҺעMÿo5-10ȥ׃(ni)ϴҺĸ˷(f)5ĸ
2ϴC(j)ϴ壺xϴ5εijϴϴҺ(yng)עMÿף_ÿoˮĸ
5. @ɫ ȼ@ɫAÿ0.05 ټ@ɫBÿ0.05ֻ37ܹ30犡
6. Kֹ(yng) ÿKֹҺ0.05
7. yø(bio)xx(sh)xβL450 nmԿհУ㣩pL450/630 nmxȡODֵx(sh)ڽKֹ(yng)10犃(ni)
ֵ
1. ԌƽֵС0.1Ԍƽֵ1.2(sh)(yn)Y(ji)Ч
2. ԌODֵ0.050.05Ӌ㣬0.05(sh)HODֵӋ
3. Cutoff ֵӋ㣺 COV = ԌƽODֵ2.1
z(yn)Y(ji)Ľጡ
1. (bio)ODֵCOV(bio)ODֵCOVԡ
2. ԽY(ji)ӱвHBsAgӱеHBsAgԇеęzy
3. ԽY(ji)ӱкHBsAgخ(yng)
4. ԽY(ji)ĘӱҪM(jn)؏(f)(sh)(yn)ֻн(jng)^؏(f)؏(f)(sh)(yn)ԞԽY(ji)ĘӱJ(rn)HBsAg؏(f)Y(ji)ԵĘӱ(yng)(jng)^m(dng)?sh)ֶM(jn)д_J(rn)
ԽY(ji)Ęӱ(f)zԽY(ji)(yng)J(rn)Ԙӱ؏(f)ԽY(ji)܌һg(sh)}£
1)ڃxӘɵĽȾ
2)@ɫxȾ
3)ԇ©ɵĽȾ
4)ֵϴȥصס
z(yn)ľԡ
иԵߌ(sh)(yn)ϵy(tng)ڝڵķخԷ(yng)؏(f)ԽY(ji)(yng)һm(dng)?sh)ķM(jn)д_J(rn)
a(chn)Ʒָ(bio)
1. ԇ^ҺwM֑(yng)
2. ԅƷʣÇ҅ƷM(jn)ЙzóF(xin)
3. ԅƷʣÇ҅ƷM(jn)Йzzy3ݝֵ5104IU/mlHBsAgԅƷóF(xin)
4. ͙zÇ҅ƷM(jn)ЙzHBsAg adradway͵͙zҪ
5. ԣþԅƷyCV%(yng)15%n=10
6. (wn)ԇ(yn)ԇM376죬zY(ji)_(d)Ϙ(bio)(zhn)
7. bݘ(bio)ʾb
ע(xing)
1. z(yn)ϴ|(zh)P(gun)ҪȱϴҺ^ܳM(yng)ϴΔ(sh)(yng)5(jng)עzҺ^Ƿ
2. ֹϴrՈʹÎмˮİԷԴ^ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zyY(ji)Ĝ(zhn)_
3. ϴrõˮՈ(f)ʹá
4. ϴC(j)ÿʹǰsˮȥxˮ_ϴɃԷֹ·g
5. κԇ|Ƥw۾ôˮԓλM(jn)ДU(ku)ϴKֹҺиg
6. ԇЃ(ni)P(gun)MݼRӱ(yng)ҕНڂȾՈP(gun)Č(sh)(yn)ҹҎ(gu)(zh)к̎
7. ʹȫԄøϵy(tng)ܕϵy(tng)`(yng)ϵy(tng)(sh)O(sh)m(dng){(dio)ԝMP(gun)|(zh)ƷҪ
ˎƷa(chn)SC̖:20160052
a(chn)Ʒ(zhn)̖ˎ(zhn)S10950045
a(chn)Ʒ(bio)(zhn)̖YBS00412010
f(zhn)ڡ